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Testosterone Test

Also known as: Testosterone Profile Test

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Testosterone Test Overview

Testosterone is synthesized by both males and females. Male sex hormones (androgens) include testosterone. Androgens aid sex organ development and other gender-linked traits. Increase in androgen levels causes increased body hair growth which may be indicative of PCOS in women. Low testosterone affects over 25% men in India and is caused by unhealthy lifestyle, including smoking, consuming alcohol and junk food, high stress levels etc. Testosterone tests can also be used to monitor the health of transgenders.

A testosterone levels test measures the amount of testosterone in a blood sample. Although testosterone is considered a "male" sex hormone, females also do have it, albeit in lower levels. Testosterone is by the testes in males and by ovaries and adrenal glands in females.

What is testosterone?

Testosterone is a steroid hormone produced primarily in the testes of males. It is responsible for the development of male secondary sexual characteristics, such as increased muscle and bone mass and body hair growth. It also plays a role in regulating male fertility and overall health and well-being.

If you’re feeling off-balance, constantly tired, or noticing unexplained changes in your body, a Testosterone Profile Test can provide valuable insights. It measures different forms of testosterone in your blood, including free, bioavailable, and total testosterone, which are vital for both men and women.

  • Evaluates hormonal health
  • Helps identify testosterone deficiency or excess
  • Assists in diagnosing conditions like PCOS, infertility, or delayed puberty
  • Monitors testosterone replacement therapy
  • Applicable to men, women, and transgender individuals
  • Quick blood test with results typically available within 8–24 hours, depending on the lab schedule
  • No fasting required, but avoid high doses of biotin at least 8 hours before collection
  • Ideal for early detection of hormonal changes

Understanding your hormone levels can guide your healthcare journey with more clarity and confidence.

Written by: Dr. Jasmin Surana, MD Biochemistry, Dept. of Biochemistry

Reasons for Undergoing the Testosterone Profile Test

You may be recommended this test if you are experiencing symptoms linked to hormonal imbalance. It is not just for men; women and adolescents may need it too.

  • Investigates low libido, fatigue, or depression
  • Detects hormone-related conditions like PCOS in women
  • Checks causes of irregular periods or excessive hair growth
  • Evaluates delayed puberty in teenagers
  • Assesses infertility issues in both genders
  • Helps manage ongoing hormone therapy
  • Screens hormonal health during health check-ups
  • Tracks recovery in post-surgery or chronic conditions

The test offers a clearer view of what’s happening inside your body when symptoms don’t have a straightforward cause.

Preparation For Testosterone Profile Test

The Testosterone Profile test preparation is usually simple and doesn’t require much effort, making the process easy and comfortable for you. However, a few small steps can help ensure your test results are accurate and meaningful.

  • Fasting is generally not required for this test unless your doctor advises otherwise.
  • Book your test in the morning, when testosterone levels are naturally at their highest.
  • Avoid intense physical activity the day before, as it may temporarily alter hormone levels.
  • Limit alcohol the evening before your test, as it can affect your hormone balance.
  • Let your doctor know about any medications, supplements, or herbal products you're currently using.
  • Stay well-hydrated and try to keep calm—stress can also impact hormone levels.
  • Wear a loose-sleeved top to make the sample collection easier and more comfortable.

By following these steps, you will help your healthcare provider get the most accurate and reliable results from your test.

Procedure of the Testosterone Profile Test

The Testosterone Profile test procedure is quick and straightforward, involving just a small blood sample. The process typically takes less than 10 minutes.

  • You will be seated comfortably, and a technician will clean the skin near your elbow.
  • A sterile needle is gently inserted into a vein to draw blood.
  • You might feel a small pinch or sting, but it’s usually brief and well-tolerated.
  • The collected blood is stored in a tube and sent to a certified pathology lab.
  • After collection, a cotton pad or bandage is applied to stop bleeding.
  • You can resume normal activities immediately unless advised otherwise.
  • The sample undergoes advanced analysis to measure various hormone levels.
  • Most labs deliver results digitally within 24–48 hours.

This safe and quick process provides essential data for understanding your hormone health.

Symptoms That May Call For Testosterone Profile Test

When your body is not producing the right amount of testosterone, subtle yet persistent symptoms can appear. These may affect your mood, physical health, or energy levels. If you’ve been feeling “off” and can’t find the reason, a Testosterone Profile test could offer helpful clarity.

  • Constant fatigue or low energy, even with proper rest
  • Reduced sex drive or erectile dysfunction in men
  • Irregular periods, acne, or excess facial hair in women
  • Unexplained weight gain or trouble building muscle
  • Hair thinning or noticeable hair loss
  • Mood changes such as irritability or feeling down
  • Memory problems or poor concentration
  • Delayed puberty or slow growth in adolescents

These signs may not always be linked to hormones, but they’re worth exploring. A quick blood test can help uncover whether hormone imbalance is part of the issue and guide you toward the right support.

Testosterone Profile Test Interpretation

Understanding your Testosterone Profile test result can feel overwhelming, but your doctor will guide you through it. The numbers will be interpreted alongside your symptoms and medical history.

  • Results are typically shown in nanograms per decilitre (ng/dL).
  • Healthy ranges vary by age, sex, and other health factors.
  • Low levels may indicate hypogonadism, pituitary dysfunction, or lifestyle-related issues.
  • High levels may signal PCOS in women or excess supplementation.
  • Mid-range or borderline values may require further testing or monitoring.

Always rely on your doctor to explain what your Testosterone Profile test result truly means in your case.

Factors That Can Affect the Testosterone Profile Test Result

Your Testosterone Profile test result may not always tell the full story unless you consider various influencing factors. Both internal health and external lifestyle choices can impact the outcome of your test.

  • Time of Day: Testosterone levels are typically highest in the early morning. That's why morning samples often provide the most accurate reflection.
  • Stress: Chronic stress elevates cortisol, a hormone that can suppress testosterone production.
  • Medications: Certain drugs like steroids, opioids, and antidepressants may interfere with hormone levels.
  • Alcohol & Smoking: Long-term use can disrupt hormone balance and reduce testosterone levels.
  • Underlying Conditions: Health issues such as liver disease, kidney problems, or thyroid disorders can affect testosterone production.
  • Age: It’s normal for testosterone to decline gradually with age.
  • Obesity: Excess fat, especially around the abdomen, can lower total and free testosterone.
  • Activity Levels: Both overtraining and a sedentary lifestyle may disrupt hormonal balance.

Always share your full health history with your doctor to help interpret your Testosterone Profile test result accurately.

Conclusion

Knowing your testosterone levels is a vital step toward better health. Whether you are facing fatigue, fertility concerns, or hormonal imbalances, this test can guide your next steps. 

For accurate, timely, and trusted results, consider Metropolis Healthcare—a leader in diagnostics with NABL and CAP-accredited labs, expert pathologists, and convenient home collection services across India.

List of Parameters Considered During the Testosterone Profile Test

The Testosterone Profile test doesn’t just measure testosterone alone—it looks at several hormones to give a full picture. These parameters help doctors better understand what might be causing your symptoms.

  • Total Testosterone: The overall testosterone present in your bloodstream.
  • Free Testosterone: The active form not bound to proteins, available for use in the body.
  • Bioavailable Testosterone: The combination of free testosterone and that loosely bound to albumin.

These markers work together to give a comprehensive view of your hormonal status.

Home Collection for Testosterone Profile Test Near You

If visiting a lab is difficult, home sample collection is a great option. It offers comfort, especially for seniors, busy professionals, or those with mobility issues.

  • Book online or through a call with a trusted diagnostic provider.
  • A certified technician will visit your home at a scheduled time.
  • All safety and hygiene protocols are strictly followed.
  • Sample collection is quick and professionally handled.
  • Digital reports are usually sent within 24–48 hours.
  • Payment can be done online or at the time of service.
  • Available across most urban and semi-urban locations.

For example, established lab networks with NABL and CAP accreditation like Metropolis Healthcare offer this reliable and stress-free service throughout India.

Testosterone Profile Test Price in Different Cities

The Testosterone Profile test price can vary depending on your city, the lab’s accreditation, and any added home service. Here is a general idea of what you might expect:

City

Approximate Cost (INR)

Mumbai

₹ 3000 to ₹ 4000

Delhi

₹ 3000 to ₹ 4000

Bengaluru

₹ 3000 to ₹ 4000

Chennai

₹ 3000 to ₹ 4000

Prices may change depending on lab offers or seasonal packages. Always confirm with your diagnostic provider for the most accurate quote.

Read More

Testosterone Profile Test Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the Testosterone Profile Test with a clear pricing structure.

The Testosterone Profile Test Price in Mumbai is ₹ 3,640 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

Testosterone test is designed to detect various hormonal problems such as  excess body hair growth and development of masculine features and irregular menstrual periods in women, infertility and decreased sex drive in men and women, erectile dysfunction and tumors of testicles in men, early or delayed puberty or virilization in boys or evaluation of infants with ambiguous genitalia 

Testosterone Profile is used to diagnose erectile dysfunction and infertility in males. This test also helps in the diagnosis of virilization (masculine physical traits), hirsutism (excess body hair), infertility, and polycystic ovary syndrome in females.

Tests include Total & Free Testosterone, SHBG, Albumin, Free androgen Index and Bioavailable testosterone. In case of abnormal values then additional tests include Prolactin, LH, FSH, TSH, DHEAS, 17-OHP and Cortisol.

A testosterone profile test measures the level of testosterone in the blood, including total testosterone, free testosterone and bioavailable testosterone. Our healthcare provider will consider our results, symptoms, and other factors to determine if our testosterone levels are within the normal range.

Testosterone test requires a blood sample. A tourniquet (elastic) band is placed tightly on the upper arm. The patient is then asked to make a fist. This helps in the build-up of blood filling the veins. The skin is disinfected before needle insertion and the blood sample is collected in vacutainer.

In men, high testosterone levels may mean a tumor in the testicles or adrenal glands and low testosterone levels indicate pituitary gland disease. 
In women, high testosterone levels may indicate cancer of the ovaries or adrenal glands or PCOS. High testosterone levels in women can also lead to male pattern baldness, deep voice and menstrual irregularities. Recent studies have also linked high testosterone levels in women to increased risk of uterine fibroids. 

The test results may show a variety of things based on age, symptoms, the results of additional tests, and any other conditions the patient may have.

In males, different conditions may cause low testosterone levels, including:

    • Chronic (long-lasting) diseases such as liver, or kidney disease
    • A pituitary gland disorder
    • Benign tumours (not cancer)
    • Damage to the testicles
    • Alcohol use disorder
    • Certain genetic diseases, such as Klinefelter syndrome

Low T levels in females are normal, but excessively low levels may indicate:

    • An adrenal gland disorder
    • A pituitary gland disorder
    • An ovarian disorder

In males, it may cause high T levels by:

    • A tumour in the testicles
    • Steroid use
    • Congenital adrenal hyperplasia (an adrenal gland disorder)

In females, it may cause high T levels by:

    • Polycystic ovary syndrome (PCOS)
    • Ovarian cancer
    • An adrenal gland disorder, such as congenital adrenal hyperplasia (CAH)
    • A disorder of the pituitary gland

If results are not normal, it does not automatically imply that we have a medical issue that requires treatment. It's common for testosterone levels to fall as we become older. Certain medications can impact our results. It is advisable to speak to a doctor in case of any apprehension.

A testosterone profile test is typically done in men who have hypogonadism (symptoms associated with low testosterone levels). Additionally, some other groups of people who may need to get a testosterone profile done are:

    • Men who are being evaluated for infertility
    • Men who have a history of testicular, pituitary, or hypothalamic disorders
    • Men who have had surgery or radiation therapy to the testicles
    • Men who have a history of anabolic steroid use
    • Men who are being evaluated for prostate cancer

A healthcare provider will consider the patient's medical history, symptoms, and physical examination before carrying out a testosterone test.

People with the following symptoms should do the Testosterone Profile test:


A testosterone profile test is often performed to evaluate symptoms of low testosterone (also known as hypogonadism) in men, which can include:

  • Low sex drive (libido)
  • Erectile dysfunction (ED)
  • Fatigue
  • Loss of muscle mass and strength
  • Increased body fat
  • Decreased bone density
  • Changes in mood, such as depression or irritability
  • Difficulty concentrating
  • Hot flashes

It's important to note that these symptoms can be caused by other conditions as well; therefore, a testosterone profile test is typically done as part of a larger evaluation that may include additional tests and a physical exam.

No special preparation is typically required for a testosterone profile test. However, there are a few things that we should keep in mind to ensure accurate results:

    • The test should be done in the morning, as testosterone levels tend to be highest in the morning and can vary throughout the day.
    • Avoid alcohol and caffeine for 24 hours before the test.
    • Avoid strenuous exercise for 24 hours before the test.
    • Avoid any medications that can affect testosterone levels.

A testosterone profile test is a useful tool for evaluating testosterone levels, but it is not a diagnostic test. Therefore, it will not tell you:

    • The cause of low testosterone levels
    • The exact cause of the problem
    • If sperm production has been affected/decreased

Therefore, a testosterone profile test should be interpreted in the context of the individual's clinical presentation, symptoms, and other laboratory tests.

Normal total testosterone results in adult men:

    • Ages 19 to 49 years: 249 - 836 ng/dL
    • Ages 50 years and older: 193 - 740 ng/dL

Normal total testosterone results in adult women:

    • Ages 19 to 49 years: 8 - 48 ng/dL
    • Ages 50 years and older: 2 - 41 ng/dL

A testosterone test may consist of two different tests:

  • Total testosterone tests the total amount of testosterone in the body, which includes both the amount linked to proteins that aid the transport of the hormone through the bloodstream as well as free testosterone.
  • Free testosterone tests testosterone that is not bound to proteins.

The test can be performed on men or women. Testosterone levels can help doctors determine fertility or menstruation disorders.

Testosterone Total Test

Total Testosterone Test

Free Testosterone Test

Sex Hormone Binding Globulin (SHBG)

Albumin

Free Androgen Index

Bioavailable testosterone

full medical check up

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