Vitamin B Complex Profile
Vitamin B Complex Profile Overview
Tests include : Vitamin B1, B2, B6, by HPLC, B9 (Folic acid), Vitamin B12 by CMIA. Fasting recommeded
Vitamin B Complex Profile is a group of tests that measure the levels of various B vitamins in the blood. B vitamins are a group of water-soluble vitamins that play important roles in the body's metabolism, energy production, and nerve function. The specific vitamins included in a Vitamin B Complex Profile test include:
- Vitamin B1 (thiamine)
- Vitamin B2 (riboflavin)
- Vitamin B6 (pyridoxine)
- Vitamin B9 (folic acid)
- Vitamin B12 (cobalamin)
Vitamin B Complex Profile is usually done to evaluate vitamin B deficiency and malabsorption issues. Low levels of certain B vitamins can lead to a variety of symptoms such as fatigue, muscle weakness, nerve damage, and anemia. The test results will help to identify which vitamin(s) is/are deficient and determine the appropriate treatment.
Written by: Supriya Kulkarni, Lead-Content & Training
Reasons for Undergoing the Vitamin B Complex Profile
The Vitamin B Complex Profile is a crucial diagnostic tool that helps assess the levels of all eight B vitamins in the body. There are several important reasons to undergo this test:
- Diagnosing deficiencies in any of the B vitamins, which can lead to various health issues such as fatigue, neurological problems, and impaired immune function.
- Investigating symptoms suggestive of B vitamin deficiencies, such as numbness or tingling in the hands and feet, weakness, or neurological symptoms.
- Screening high-risk groups, including individuals with conditions that increase the risk of B vitamin deficiencies, such as celiac disease, Crohn's disease, or those who have undergone weight loss surgery.
- Ensuring adequate levels of B vitamins, especially folate and vitamin B12, during pregnancy for fetal health and preventing birth defects like neural tube defects.
- Monitoring the effectiveness of treatment for B vitamin deficiencies and adjusting supplement dosages accordingly.
Preparation For Vitamin B Complex Profile Test
Getting ready for a Vitamin B Complex Profile test is simple and nothing to worry about. Preparing properly helps ensure that your results are as accurate and helpful as possible. Here is how you can get ready with confidence:
- Fasting may be recommended: You may be asked to fast for 8–10 hours before your test. Please follow your doctor’s advice carefully. Water is usually allowed.
- Stay hydrated: Drinking water before the test helps with blood collection and ensures smoother sample withdrawal.
- Inform your doctor about medications: Some medicines can affect vitamin levels. Let your healthcare provider know what you’re taking.
- Avoid supplements beforehand: If you are on vitamin supplements, your doctor might advise pausing them before the test.
- Dress comfortably: Wear loose sleeves to allow easy access for the blood draw.
These simple steps form part of your Vitamin B Complex Profile test preparation and help make your experience stress-free.
Procedure of the Vitamin B Complex Profile Test
The procedure for the Vitamin B Complex Profile test is quick, safe, and performed by trained professionals. You don’t need to feel anxious — it is a routine test done regularly at labs and hospitals.
- Blood sample collection: A small amount of blood is drawn from a vein in your arm, usually using a sterile needle and vial.
- Minimal discomfort: You may feel a slight pinch during the needle insertion, but it lasts only a few seconds.
- Home collection available: Many labs offer a home collection service for your comfort and convenience.
- Sample handling: Your sample is labelled and securely transported to the laboratory.
- Advanced testing methods: High-quality methods like HPLC and CMIA are used to analyse B-vitamin levels.
This is what the Vitamin B Complex Profile test procedure typically involves — simple, efficient, and over in just a few minutes.
Symptoms That May Call For Vitamin B Complex Profile Test
If you have been feeling off lately and can’t pinpoint why, it might be time to check your vitamin levels. Certain signs can point to deficiencies that a Vitamin B Complex Profile test can identify.
- Fatigue or low energy
- Numbness or tingling
- Memory problems or confusion
- Frequent mood changes or irritability
- Pale skin or anaemia symptoms
Vitamin B Complex Profile Test Interpretation & Clinical Utility:
Once your test is done, understanding the results may feel overwhelming. But don’t worry — your doctor will help you interpret them and explain what they mean for your health.
Vitamin B12 level: Vitamin B12, also known as cobalamin, is a water-soluble vitamin that plays a crucial role in various bodily functions. It is essential for maintaining healthy nerve cells, producing red blood cells, and synthesizing DNA. Vit B12 levels are decreased in megaloblastic anemia, partial/total gastrectomy, pernicious anemia, peripheral neuropathies, chronic alcoholism, senile dementia, and treated epilepsy.
Folic Acid: Low levels are seen in megaloblastic anemia, malnutrition, liver disease, chronic haemodialysis, alcoholism, and inadequate dietary intake. Low levels are associated with a higher risk of fetal malformations during pregnancy.
Vitamin B1 ( Thiamine ): Measurement of Vitamin B1 is useful in the assessment of thiamine deficiency, in the investigation of suspected beriberi, and in monitoring the effects of chronic alcoholism. Vitamin B1 is increased in leukemia, polycythemia vera, and Hodgkin's disease. It is decreased in alcoholism with and without liver disease., chronic febrile infections, prolonged diarrhoea, diabetes, carcinoid syndrome, Hartnup disease, and pellagra.
Vitamin B2 (Riboflavin): Ariboflavinosis (dietary deficiency of riboflavin) causes a syndrome characterized by sore throat, hyperemia, and edema of the pharyngeal and oral mucous membranes, cheilitis, angular stomatitis, glossitis, corneal vascularization, dyssebacia with dermatitis, and normochromic, normocytic anemia. Severe deficiency can diminish conversion of Vitamin B6 to its coenzyme and curtail conversion of tryptophan to niacin.
Vitamin B6 (pyridoxal phosphate): It acts as a coenzyme for many enzymes in the body & is involved in metabolism of protein, carbohydrates & fats, production of Serotonin & haem & binding of steroid hormones to their receptors. The common cause of deficiency is ingestion or use of antagonists like cyclosporine, hydrazaline etc. Sideroblastic anaemias have been described that are responsive to Vitamin B6 administration. It may be decreased in pregnancy, lactation, alcoholism & diabetes mellitus
Your Vitamin B Complex Profile test report will provide a clear picture of your vitamin status and help plan treatment if needed.
Factors That Can Affect the Vitamin B Complex Profile Test Result
Various things can influence your test outcomes. Being aware of these helps you and your doctor make sense of the numbers in the report.
- Recent supplements: Taking multivitamins before the test can skew your results.
- Medical conditions: Issues like liver disease or gastrointestinal problems affect vitamin absorption.
- Medications: Certain drugs (e.g., metformin or anticonvulsants) can impact B vitamin levels.
- Alcohol intake: Excessive drinking interferes with B vitamin absorption.
- Diet: Inadequate intake of vitamin-rich foods may reflect in your test.
If any of these apply to you, discuss them with your doctor. They may influence your Vitamin B Complex Profile test result, so full transparency helps ensure accuracy.
Conclusion
The Vitamin B Complex Profile test is a valuable tool in identifying hidden deficiencies that may be affecting your energy, mood, or nerve function. Early testing can make a big difference in how you feel day-to-day.
For a smooth, accurate, and convenient testing experience, Metropolis Healthcare is a trusted choice — offering NABL and CAP-accredited labs, expert staff, and home collection services across India.
List of Parameters Considered During the Vitamin B Complex Profile Test
The Vitamin B Complex Profile measures the levels of all eight B vitamins in the body, each playing a crucial role in various bodily functions. The parameters included in the test are:
- Vitamin B1 (Thiamine): Indicates energy metabolism, nerve health, and muscle function. Deficiency can lead to beriberi and Wernicke-Korsakoff syndrome.
- Vitamin B2 (Riboflavin): Indicates antioxidant activity, energy production, skin, and mucous membrane health. Deficiency can cause skin lesions and eye problems.
- Vitamin B3 (Niacin): Indicates energy production, skin health, and cholesterol levels. Deficiency leads to pellagra.
- Vitamin B5 (Pantothenic Acid): Indicates energy production, hormone synthesis, and cholesterol production. Deficiency is rare but affects energy metabolism.
- Vitamin B6 (Pyridoxine): Indicates brain development, neurotransmitter synthesis, immune function, and red blood cell formation. Deficiency can lead to neurological disorders and weakened immune function.
- Vitamin B7 (Biotin): Indicates fatty acid synthesis, glucose metabolism, skin, hair, and nail health. Deficiency is rare but can affect these areas.
- Vitamin B9 (Folate/Folic Acid): Indicates DNA synthesis, red blood cell formation, and fetal development. Deficiency can lead to megaloblastic anaemia.
- Vitamin B12 (Cobalamin): Indicates nerve function, red blood cell formation, and DNA synthesis. Deficiency can result in megaloblastic anaemia and neurological problems.
Home Collection for Vitamin B Complex Profile Test Near You
Metropolis Healthcare offers a convenient home sample collection option for the Vitamin B Complex Profile test, ensuring comfort and safety for patients. A trained phlebotomist will visit your home to collect the required blood samples, maintaining high standards of sample handling and testing accuracy.
This service also eliminates your need to visit the diagnostic center, as it is easier for individuals having mobility issues or busy schedules to access quality healthcare. With Metropolis Healthcare's commitment to providing reliable and timely results, you can trust that your Vitamin B Complex Profile will be analyzed with precision and care. By bringing diagnostic solutions to your doorstep, Metropolis Healthcare aims to make healthcare more accessible and convenient for everyone.
Vitamin B Complex Profile Price
Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the Vitamin B Complex Profile with a clear pricing structure.
The Vitamin B Complex Profile Price in Rajkot is ₹ 6,420 .
We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.
Frequently Asked Questions
A Vitamin B Complex Profile test is typically ordered for the following reasons:
- To evaluate for deficiency: A deficiency in one or more of the B vitamins can lead to a variety of symptoms such as fatigue, muscle weakness, nerve damage, and anemia. The test results can help identify which vitamin(s) is/are deficient and determine the appropriate treatment.
- To monitor treatment of deficiency: After a deficiency has been identified, the test can be used to monitor the effectiveness of treatment. The levels of the deficient vitamin(s) should increase with proper treatment.
- To evaluate malabsorption issues: Malabsorption of nutrients can lead to deficiencies in vitamins, including B vitamins. The test results can help identify malabsorption issues and determine the appropriate treatment.
- To evaluate for certain medical conditions: Certain medical conditions such as pernicious anemia, which affects the body's ability to absorb Vitamin B12, require specific Vitamin B12 level monitoring.
A Vitamin B Complex Profile test is typically ordered for people who have symptoms of deficiency or malabsorption issues or have certain medical conditions that require monitoring of specific B vitamins level.
The following individuals may be at higher risk of B vitamin deficiencies and may benefit from a Vitamin B Complex Profile test:
- People with malabsorption issues, such as those with celiac disease or Crohn's disease, as these conditions can affect the body's ability to absorb nutrients.
- Vegetarians and vegans, as they may be at higher risk of B12 deficiency.
- Alcoholics, as alcohol consumption can interfere with the absorption and metabolism of B vitamins.
- People taking certain medications, such as metformin for diabetes, as these medications can affect the levels of B vitamins in the body.
- People with anemia, as low levels of certain B vitamins, such as folic acid and B12, can lead to anemia.
- Pregnant women and women who are planning to become pregnant, as a deficiency in folic acid can lead to neural tube defects in the developing fetus.
- People with symptoms like fatigue, muscle weakness, nerve damage, anemia, skin rashes, cracking of the corners of the mouth, sore throat, eye disorders, and dementia.
Vitamin B Complex Profile measures the level of various B vitamins in the blood viz. vitamin B1, B2, B6, B9 (Folic acid), and Vitamin B12.
This test requires a blood sample. A tourniquet (elastic) band is placed tightly on the upper arm. The patient is then asked to make a fist. This helps in the build-up of blood filling the veins. The skin is disinfected before needle insertion and the blood sample is collected in vacutainer.
If Vitamin B Complex Profile test results are abnormal for Vitamin B1, B2, B6, B9 (Folic acid), and Vitamin B12, it means that the levels of these vitamins are outside the normal range. This suggests that the individual may have a deficiency in one or more of these vitamins.
Associated Tests:
- Vitamin Profile
- Vitamin B1 (Thiamine)
- Vitamin B2 Riboflavin
- Vitamin B6 Pyridoxine
- Folic Acid Serum
- RBC Folate EDTA Whole Blood
- Vitamin B12 Active Holo Transcobalamin
- Vitamin B12 Cyanocobalamin Serum
Ratings & Reviews (0)
Why Metropolis?
Metropolis has a team of 200 senior pathologists and over 2000 technicians delivering diagnostic solutions in the areas of routine, semi specialty and super specialty domains like Oncology, Neurology, Gynaecology, Nephrology and many more.
We offer a comprehensive range of 4000+ clinical laboratory tests and profiles, which are used for prediction, early detection, diagnostic screening, confirmation and/or monitoring of the disease.

WhatsApp