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Vitamin Profile Test

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Vitamin Profile Test Overview

Vitamin D is crucial for the absorption of calcium, and a lack of it can cause discomfort, weak bones, and irregular bone growth. Additionally, it aids in keeping parathyroid hormone, calcium, and phosphate levels in check. PTH, a parathyroid hormone, regulates blood calcium levels. The vitamin profile test is frequently carried out to determine the cause of osteoporosis, kidney stones, hyper- or hypocalcemia, and low vitamin D levels. The purpose of the vitamin profile is to evaluate the condition of other nutrients, such as vitamin B12, calcium, and magnesium, which are frequently impacted when levels are aberrant.

Created by: Dr.Shibani R Medical Writer, Medical Affairs

Reasons for Undergoing the Vitamin Profile Test

There are several compelling reasons to undergo a vitamin profile test:

  • If you are experiencing symptoms that could indicate a vitamin deficiency, such as fatigue, muscle weakness, bone pain, or neurological issues, your doctor may recommend a vitamin profile test.
  • Individuals with certain conditions or lifestyles that increase the risk of vitamin deficiencies, such as celiac or Crohn's disease, malabsorption syndrome, alcohol use disorder, limited sun exposure, darker skin pigmentation, obesity, or a plant-based diet (vegetarian or vegan), may need a vitamin profile test.
  • To check the effectiveness of vitamin supplements or to monitor vitamin levels in individuals who are already taking supplements
  • As part of a routine health screening to identify potential nutritional deficiencies before they lead to serious health issues

Preparation For Vitamin Profile Test

Getting ready for your Vitamin Profile test is simple and stress-free. You don’t need to worry about any complicated steps, but being informed helps make the experience smoother.

Here are a few things to keep in mind for yourVitamin Profile test preparation:

  • No fasting is required: You can eat and drink normally before the test unless your doctor advises otherwise.
  • Inform your doctor about medications: Certain supplements or medicines might affect vitamin levels, so always let your healthcare provider know what you're taking.
  • Stay hydrated: Drink water before the test, as it can make the blood draw easier.
  • Wear comfortable clothing: Especially one that allows easy access to your arm for the blood sample.
  • Stay calm: The test is routine and usually completed within minutes. Being relaxed helps ease the process.

Procedure of the Vitamin Profile Test

The Vitamin Profile test sample collection procedure is straightforward and typically completed within a few minutes. It is designed to be as comfortable and convenient as possible.

Here is how it works:

  • Sample collection: A trained technician draws a small amount of blood from a vein in your arm.
  • No discomfort expected: You may feel a brief pinch, but it's usually painless.
  • Home collection option: Many diagnostic labs like Metropolis Healthcare offer home sample collection to make things even easier.
  • Sample handling: The blood is securely transported to the lab where it’s analysed for key vitamins like D, B12, and others.
  • Report delivery: Results are often available within 24 hours and shared digitally or in person.

The whole process is safe, quick, and conducted using hygienic, certified methods.

Symptoms That May Call For Vitamin Profile Test

Sometimes, your body gives subtle signals that could point towards vitamin deficiencies. A Vitamin Profile test can help identify if something is off and offer timely insights.

Here are common symptoms that might suggest the need for this test:

  • Persistent fatigue or tiredness
  • Muscle weakness or cramps
  • Bone pain or softening
  • Mood changes or irritability
  • Numbness or tingling in limbs
  • Poor wound healing or frequent infections

If you notice any of these, don’t panic—but do speak with your doctor and consider a Vitamin Profile test for clarity.

Vitamin Profile Test Interpretation & Clinical utility :

Vitamin Profile test is a combination of test comprising of Vitamins, hormone & minerals which are commonest nutritional deficiency & are involved in metabolic disorders. This test helps in dietary planning & targeted supplementation. The test parameters are –

Ionised Calcium : Ionised calcium is the preferred measurement rather than total calcium because it is physiologically active and can be rapidly measured. Useful in diagnosing patients with hypocalcemia or hypercalcemia with borderline serum calcium and altered serum proteins.

Low levels are seen commonly in primary hypoparathyroidism, vitamin D deficiency,  renal disorders, malabsorption, Osteomalacia, Pancreatitis, and rickets

High levels are seen in primary hyperparathyroidism, vitamin D therapy, various malignancies  producing parathyroid hormone, Acidosis, Metastatic bone tumor, Milk-alkali syndrome, Multiple Myeloma Paget disease, and Sarcoidosis.

Magnesium :

Magnesium is a mineral that is important for immunity, muscle contraction, nerve function, strong bones and regulating  blood pressure as well as blood sugar.

High levels of magnesium may result from taking too many supplements/antacids/laxatives that contain magnesium, kidney  disease, Addison disease, dehydration and diabetic ketoacidosis. Low levels of magnesium may be seen in chronic diarrhea, hemodialysis, gastrointestinal disorders, cirrhosis,  hyperaldosteronism, hypoparathyroidism, severe burns, pancreatitis, preeclampsia, ulcerative colitis and uncontrolled diabetes

Vitamin D & Parathyroid hormone (PTH) :

Vitamin D and PTH play crucial roles in calcium and phosphate homeostasis, and their interplay is essential for bone health. Vitamin D regulate calcium absorption in the intestines and bone resorption, increasing calcium and phosphate levels in the blood. PTH, secreted by the parathyroid glands, also regulates calcium and phosphate levels.  For diagnosis of vitamin D deficiency it is recommended to have clinical correlation with serum vitamin D, serum calcium & serum PTH. An inverse relationship exists between PTH and Vitamin D levels.

Holotranscobalamin (Active Vitamin B12) :  

This test helps evaluate the functional, active form of vitamin B12, which is crucial for proper bodily functions. It specifically measures the vitamin B12 that is actively transported into cells, making it a key indicator of B12 availability. Unlike total vitamin B12 tests, which measure all forms of Vitamin B12, the Holotranscobalamin test provides a more direct measure of the B12 that is actually being used by the body. Low levels can indicate a deficiency, even if total B12 levels are within the normal range. The test can be used to track the effectiveness of B12 supplementation or treatment for deficiency.

Always consult your healthcare provider to understand your Vitamin Profile test result in context of your overall health.

Factors That Can Affect the Vitamin Profile Test Result

Several external and internal factors can influence the outcome of your Vitamin Profile test. Being aware of them ensures you get the most accurate and meaningful insights.

Here is what might affect your results:

  • Recent supplements or multivitamins: These can temporarily alter vitamin levels in your blood.
  • Medications: Some drugs can interfere with how your body absorbs or processes vitamins.
  • Underlying medical conditions: Conditions like Crohn’s disease or kidney disorders may impact vitamin absorption.
  • Lifestyle factors: Smoking, alcohol use, or a highly restrictive diet can skew results.
  • Time of year and sun exposure: Vitamin D levels are affected by seasonal changes and how much sunlight you receive.

If you are concerned about anything possibly affecting yourVitamin Profile test result, speak with your doctor for tailored advice.

Conclusion

Staying ahead of your health starts with understanding what your body needs. A Vitamin Profile test helps detect deficiencies early, so you can take the right steps with confidence. For accurate reports, expert support, and home collection convenience, trust Metropolis Healthcare—a leader in diagnostics with 40+ years of experience and NABL & CAP-accredited labs nationwide.

List of Parameters Considered During the Vitamin Profile Test

During a vitamin profile test, several key parameters are measured:

  • Vitamin D: This vitamin is crucial for bone health, muscle function, and immune system integrity. The vitamin profile test measures 25-hydroxyvitamin D (25(OH)D) levels, which indicate the body's vitamin D status. Low levels can suggest deficiencies, malabsorption, or kidney/liver issues.
  • Vitamin B12: This vitamin is essential for energy metabolism, blood cell formation, and nervous system function. Deficiencies can lead to fatigue, neurological problems, and certain types of anaemia.
  • Other B Vitamins: Depending on the test, other B vitamins such as thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxal phosphate (B6), biotin (B7), and folate (B9) may be measured. Each B vitamin has distinct roles in metabolism, blood cell production, and nervous system health.
  • Vitamin E: Though less commonly included, vitamin E is important for antioxidant functions and overall health. Deficiencies can lead to neurological problems and impaired immune function.

Home Collection for Vitamin Profile Test Near You

Choosing Metropolis Healthcare's home collection service for your vitamin profile test comes with several advantages. A trained phlebotomist will visit your home at a time that suits you, ensuring both comfort and safety while collecting your blood sample.

Metropolis Healthcare maintains high standards of sample handling protocols, ensuring that the accuracy of your vitamin profile test is never compromised. You can expect to receive timely and accurate results, as Metropolis Healthcare is dedicated to enhancing healthcare accessibility and providing high-quality diagnostic solutions directly at your doorstep.

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Vitamin Profile Test Price

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the Vitamin Profile Test with a clear pricing structure.

The Vitamin Profile Test Price in Rajkot is ₹ 2,995 .

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

 

  • Low vitamin D levels can help diagnose a case of  secondary hyperparathyroidism.
  •  PTH test is most often used along with vitamin D levels to diagnose hypoparathyroidism  & monitor kidney disease.
  • It is also used to diagnose cause for osteoporosis & kidney stones.
  • Vitamin B12, calcium and magnesium levels are also important for efficient functioning of nerves, muscles and even cardiac health.

Doctors frequently prescribe a vitamin profile test for patients exhibiting any of the below-listed symptoms that cannot be adequately diagnosed or treated:

  • Anorexia or significant weight loss.
  • Hair loss.
  • Fatigue.
  • Inability to stand up straight after eating.
  • Bone pain brought on by cytokine-mediated inflammation (increased CRP).
  • Elevated liver enzymes (such as AST/ALT) with normal bilirubin levels.
  • Ultrasound-confirmed hepatomegaly, or an enlarged liver.

In addition to the vitamin profile test, a few more tests like the following will also be ordered:

  • TSH.
  • Thyroid-stimulating hormone.
  • Ionized calcium.
  • Magnesium.
  • Folic acid or folates.
  • Vitamin A.
  • Vitamin profile test is used to diagnose vitamin D, vitamin B12 and micronutrient deficiency in the blood.
  •  It measures 25 hydroxy vitamin D levels. If the levels are abnormal, PTH levels in blood are tested along with active vitamin B12, calcium and magnesium levels.
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